vs. S.Aureus
vs. S.Maltophilia
To find all the exact matches it is adviseable to break the CFTR protein into small fragments (e.g. 15 amino acids overlapping by 5 for each consecutive BLAST).

Autoantigens

CFTR BLAST

vs. Adrenergic beta receptor 2 ADBR2
vs.Bactericidal/permeability-increasing protein BPI NP_001716.2
vs. Calgranulin B (S100A9)
vs.Glutamate decarboxylase GAD2 (GAD65)
vs.Heat shock protein 60 (HSPD1)
vs.Mucin 1 (tracheal)
vs Myeloperoxidase MPO
vs.Proteinase 3 (PRTN3)
vs.Rheumatoid factor
vs.Tumour necrosis factor alpha

BLAST results showing the consensus sequences along the length of the native CFTR protein for S.Aureus, P.Aeruginosa, S.Maltophilia or autoantigen matches: The B cell and T cell epitope immunogenicity index is also provided. Values above the server-set thresholds (0.35 for B cell and 0.5 for T cell ) are shown in red. The topology of the CFTR protein is shown in the final column (EXT = extracellular: IN = intracellular; TM = transmembrane): Extracellular regions are highlighted in red: Autoantigen overlaps are highlighted in blue. The whole protein is not processed and BLAST searches were focussed on auto antigen overlaps in antigenic extracellular regions.

Jens Erik Pontoppidan Larsen, Ole Lund and Morten Nielsen. Improved method for predicting linear B-cell epitopes. Immunome Res. 2006; 2: 2.

M. Nielsen, C. Lundegaard, O. Lund, and C. Kesmir. 2005. The role of the proteasome in generating cytotoxic T cell epitopes: Insights obtained from improved predictions of proteasomal cleavage. Immunogenetics. 57:33-41.

BLAST results of the F508del and 19 other mutants are shown at the bottom of this page

N

Autoantigen coverage in blue

Pathogen or autoantigen alignment

B cell epitopes in red

T cell epitopes in red

Topology (Extracellular= Red)

1

M

S.Malt MQR PL  AS

0.64

0.84

IN

2

Q

Q

0.36

0.10

IN

3

R

(mucin) RSP EK S P.Aer R PLEK S and RSPLE  KA  and RSPL KA S.Malt RSPL+K S.Aur R P LEKA

0.61

0.59

IN

4

S

S

0.65

0.03

IN

5

P

S.Aur and S.Malt PLEKA

0.60

0.02

IN

6

L

L

0.83

0.40

IN

7

E

S.Malt LEKAS

0.63

0.03

IN

8

K

K

0.36

0.30

IN

9

A

A

0.19

0.09

IN

10

S

S

0.07

0.04

IN

11

V

V

-0.17

0.27

IN

12

V

V

-0.75

0.94

IN

13

S

S

-1.19

0.06

IN

14

K

K

-1.17

0.84

IN

15

L

L

-1.48

0.96

IN

16

F

F

-1.14

0.82

IN

17

F

F

-0.90

0.15

IN

18

S

S

-0.89

0.03

IN

19

W

W

-1.07

0.93

IN

20

T

T

-0.94

0.03

IN

21

R

R

-0.60

0.58

IN

22

P

(myelo) PILR  P.Aer and   S.Aur and S.Malt PILRK S.Malt P+LR GYRQ

-0.20

0.10

IN

23

I

I

-0.15

0.49

IN

24

L

(S.Malt) LRK Y QR

0.07

0.66

IN

25

R

R

-0.06

0.55

IN

26

K

(P.Aer)KGYR

-0.16

0.95

IN

27

G

S.Malt  GYRQRL

-0.15

0.07

IN

28

Y

(P.Aer) YRQRL

-0.25

0.92

IN

29

R

(P.Aer) RQRLE-SD

0.03

0.84

IN

30

Q

(S.Malt) QRL LSD

-0.21

0.08

IN

31

R

(P.Aer) RLEL

-0.19

0.57

IN

32

L

L

-0.18

0.98

IN

33

E

E

-0.35

0.02

IN

34

L

L

-0.57

0.71

IN

35

S

S

-0.49

0.04

IN

36

D

(P.Aer) DIYQI

-0.64

0.03

IN

37

I

I

-0.26

0.10

IN

38

Y

Y

-0.13

0.97

IN

39

Q

Q

0.18

0.05

IN

40

I

I

0.33

0.70

IN

41

P

P

0.40

0.17

IN

42

S

(P.Aer) SVDSA-N

0.70

0.05

IN

43

V

V

1.04

0.82

IN

44

D

D

1.08

0.23

IN

45

S

S

1.02

0.10

IN

46

A

A

1.06

0.13

IN

47

D

D

0.95

0.07

IN

48

N

(P.Aer) +NLSE

1.01

0.10

IN

49

L

(myelo) LSEK +P.Aer and  S.Aur and S.Malt LSEKL

0.54

0.68

IN

50

S

S

0.50

0.06

IN

51

E

S.Malt EKLERE

0.43

0.04

IN

52

K

K

0.44

0.85

IN

53

L

L

0.12

0.97

IN

54

E

E

0.56

0.02

IN

55

R

R

0.49

0.26

IN

56

E

E

0.56

0.03

IN

57

W

W

0.22

0.53

IN

58

D

D

0.45

0.02

IN

59

R

(P.Aer) RELAS

0.43

0.75

IN

60

E

E

0.53

0.03

IN

61

L

L

0.56

0.95

IN

62

A

A

1.00

0.16

IN

63

S

S

0.84

0.18

IN

64

K

K

0.89

0.60

IN

65

K

K

0.56

0.31

IN

66

N

N

0.53

0.03

IN

67

P

P

0.51

0.02

IN

68

K

K

0.31

0.28

IN

69

L

L

-0.11

0.80

IN

70

I

I

-0.43

0.19

IN

71

N

(P.Aer) NALR

-0.83

0.03

IN

72

A

A

-1.11

0.15

IN

73

L

L

-1.61

0.82

IN

74

R

(prot) RRC F   F F  P.Aer  RRCFF S.Aur RCFF S.Malt RR FF

-1.81

0.69

IN

75

R

R

-1.92

0.11

IN

76

C

C

-2.00

0.03

IN

77

F

F

-2.28

0.05

IN

78

F

(P.Aer) FWRF+ Y

-2.29

0.06

IN

79

W

(P.Aer) WRFM (rheum) WRF+F

-2.56

0.11

IN

80

R

R

-2.62

0.05

IN

81

F

F

-2.46

0.89

IN

82

M

(P.Aer) MFYG

-2.40

0.09

IN

83

F

(BPI) F+G F  L EV K

-2.34

0.11

IN

84

Y

Y

-2.43

0.85

TM

85

G

G

-2.54

0.06

TM

86

I

I

-2.46

0.24

TM

87

F

S.Malt FLYLG 

-2.12

0.34

TM

88

L

(P.Aer) LYLG+VT

-1.65

0.63

TM

89

Y

Y

-1.50

0.96

TM

90

L

(myelo)LGEV      L L 

-1.35

0.97

TM

91

G

G

-0.97

0.13

TM

92

E

E

-0.63

0.02

TM

93

V

(BPI) VT AV P

-0.17

0.86

TM

94

T

T

0.18

0.03

TM

95

K

K

0.46

0.97

TM

96

A

A

0.14

0.80

TM

97

V

V

-0.24

0.92

TM

98

Q

(P.Aer) QPLL-GRI

-0.57

0.03

TM

99

P

P

-0.72

0.04

TM

100

L

L

-0.84

0.94

TM

101

L

S.Malt LLGR+I S

-1.09

0.78

TM

102

L

L

-1.36

0.20

TM

103

G

G

-1.58

0.05

TM

104

R

R

-1.46

0.21

TM

105

I

I

-1.09

0.61

TM

106

I

I

-0.49

0.54

TM

107

A

S.Malt A YDPDN E   ERS+ and A YDPD S.Aur ASYD and A YDPD S.Malt ASYDP   DN and  AS DPDN P.Aer A YDPD and ASYD D and ASYD P   EE

0.07

0.72

EXT

108

S

P.Aer SYDPD    N E R S.Aur SY DP++KE  and SY D DNKE  and SYDP   EE S.Malt  SYDPD

0.49

0.09

EXT

109

Y

(prot) YD +NK (P.Aer) YDPD and  YD   KEER S.Aur  Y PD NKEE and YD +NKE+ and  YDPD  EE and  YD +NKE+ and YDP   DNK

0.87

0.98

EXT

110

D

S.Aureus DPDNKE and DP++ EER and +PD KEE and D KEER  and DPD NKE  and D DNKE S.Malt DPDN  ER

1.31

0.02

EXT

111

P

(myelo) PD  +ERS S.Malt  P+ KEER and PDNK+ R P.Aer PDNKE  and P+NK ER S.Aur PD KEER

1.77

0.26

EXT

112

D

S.Aur DNKE+R and DNKEE P.Aer D KEER

2.06

0.03

EXT

113

N

S.Aur and S.Malt NKEER P.Aer NK+ER

2.02

0.03

EXT

114

K

K

2.03

0.27

EXT

115

E

E

1.42

0.08

EXT

116

E

E

1.14

0.03

EXT

117

R

R

0.41

0.38

EXT

118

S

S

-0.05

0.04

EXT

119

I

(S.Aur) IAI LG G S.Malt IAI LGIG

-0.61

0.11

EXT

120

A

(GAD65) A  LGIG

-0.77

0.03

EXT

121

I

I

-1.24

0.32

TM

122

Y

Y

-1.31

0.69

TM

123

L

(GAD65) LGIG (S.Malt) LGIGL

-1.83

0.76

TM

124

G

G

-1.76

0.06

TM

125

I

I

-2.19

0.40

TM

126

G

G

-2.35

0.03

TM

127

L

S.Malt LCLLF

-2.68

0.17

TM

128

C

C

-2.71

0.03

TM

129

L

L

-2.98

0.76

TM

130

L

(myelo) L    TLLL

-2.78

0.81

TM

131

F

F

-2.85

0.12

TM

132

I

S.Malt IVRTLL

-2.82

0.15

TM

133

V

V

-3.02

0.40

TM

134

R

R

-2.81

0.57

TM

135

T

T

-2.30

0.07

TM

136

L

(P.Aer) LLLH-A

-1.82

0.91

TM

137

L

L

-1.43

0.97

TM

138

L

L

-1.33

0.79

TM

139

H

H

-1.58

0.03

TM

140

P

P

-1.56

0.05

TM

141

A

A

-1.48

0.38

TM

142

I

I

-1.15

0.39

TM

143

F

F

-0.93

0.89

TM

144

G

G

-1.22

0.03

IN

145

L

L

-1.19

0.96

IN

146

H

H

-1.39

0.15

IN

147

H

(P.Aer)  HIGM

-1.21

0.18

IN

148

I

I

-1.14

0.58

IN

149

G

G

-1.13

0.05

IN

150

M

M

-1.16

0.35

IN

151

Q

Q

-1.23

0.03

IN

152

M

M

-1.37

0.96

IN

153

R

R

-1.43

0.04

IN

154

I

I

-1.55

0.23

IN

155

A

A

-1.72

0.03

IN

156

M

(mucin) MF L IYK

-2.06

0.05

IN

157

F

F

-1.98

0.39

IN

158

S

S

-1.90

0.02

IN

159

L

L

-1.52

0.57

IN

160

I

I

-1.31

0.32

IN

161

Y

Y

-1.38

0.95

IN

162

K

K

-1.02

0.69

IN

163

K

(P.Aer)  KT KLSS

-1.23

0.97

IN

164

T

T

-0.77

0.04

IN

165

L

(rheum) LKLSS S.Aur amd P.Aer LKLSS S.Malt LKLS

-0.44

0.92

IN

166

K

(P.Aer)  KLSS (rheum) KLSS

-0.28

0.67

IN

167

L

L

-0.48

0.97

IN

168

S

S

-0.80

0.09

IN

169

S

S

-0.79

0.03

IN

170

R

R

-0.44

0.65

IN

171

V

V

-0.69

0.44

IN

172

L

L

-0.33

0.90

IN

173

D

D

-0.62

0.03

IN

174

K

K

-0.61

0.55

IN

175

I

I

-0.59

0.39

IN

176

S

(P.Aer)  SIG LVS

-0.67

0.02

IN

177

I

I

-0.60

0.46

IN

178

G

G

-0.77

0.22

IN

179

Q

Q

-1.14

0.32

IN

180

L

(P.Aer) +LVSLLS

-1.19

0.61

IN

181

V

VSLL

-1.22

0.42

IN

182

S

S.Malt SLLSN+

-0.95

0.08

IN

183

L

L

-0.88

0.83

IN

184

L

(P.Aer) LSNNL

-1.12

0.84

IN

185

S

(rheum) SNNL

-0.83

0.03

IN

186

N

N

-0.57

0.04

IN

187

N

N

-0.67

0.03

IN

188

L

L

-0.11

0.95

IN

189

N

N

0.31

0.04

IN

190

K

K

0.41

0.76

IN

191

F

(P.Aer) FDE-LA [FDEG]

0.09

0.96

IN

192

D

(P.Aer) DEGL

-0.09

0.02

IN

193

E

(P.Aer) +GLALAH

-0.19

0.03

IN

194

G

G

-0.35

0.02

IN

195

L

(P.Aer) LALAHF

-0.52

0.81

IN

196

A

A

-0.64

0.04

TM

197

L

L

-1.13

0.79

TM

198

A

A

-1.59

0.72

TM

199

H

H

-1.90

0.03

TM

200

F

F

-1.64

0.27

TM

201

V

V

-1.55

0.13

TM

202

W

(myelo) W+ PLQV S.Malt  WIAPL +AL

-1.40

0.92

TM

203

I

S.Malt IAPLQVA

-1.16

0.14

TM

204

A

A

-1.25

0.06

TM

205

P

(GAD65) PLQ   L  L+ E  L+Q

-1.07

0.03

TM

206

L

(P.Aer) L+VALL

-1.23

0.97

TM

207

Q

S.Malt  QVALLM  I

-1.24

0.04

TM

208

V

(P.Aer) V LLMG [VALL](S.Malt) VALLM L

-1.38

0.86

TM

209

A

(P.Aer) A-LMGL

-1.46

0.05

TM

210

L

L

-1.84

0.73

TM

211

L

(P.Aer) LMGLI

-1.88

0.85

TM

212

M

(S.Malt) MG I ELLQ

-2.38

0.82

TM

213

G

G

-2.16

0.05

TM

214

L

L

-2.34

0.23

TM

215

I

I

-2.21

0.08

TM

216

W

S.Malt WELLQAS S.Aur W+LLQ P.Aer WELL+

-1.84

0.56

EXT

217

E

S.Malt  ELLQA

-1.53

0.02

EXT

218

L

L

-1.33

0.96

EXT

219

L

L

-1.08

0.91

TM

220

Q

(P.Aer) QAS-AFCG+G [QASAF]

-1.12

0.05

TM

221

A

A

-0.89

0.64

TM

222

S

S

-0.88

0.23

TM

223

A

A

-0.90

0.25

TM

224

F

(prot) FCG G LI

-0.60

0.45

TM

225

C

C

-1.02

0.03

TM

226

G

(myelo) LG L V   FQ   GR ++ + +          R   I   L      SEM E (P.Aer) GLGFLIV [GLGFL VL ]

-1.40

0.03

TM

227

L

L

-1.96

0.26

TM

228

G

G

-2.17

0.03

TM

229

F

(myelo) +L V AL

-2.31

0.08

TM

230

L

(Adren)  LIVLA (mucin) +LI LA

-2.38

0.24

TM

231

I

I

-2.74

0.17

TM

232

V

V

-2.71

0.22

TM

233

L

(P.Aer) LALF+A

-2.66

0.89

TM

234

A

A

-2.31

0.68

TM

235

L

L

-1.80

0.88

TM

236

F

F

-1.55

0.93

TM

237

Q

Q

-1.19

0.03

TM

238

A

A

-0.85

0.20

TM

239

G

G

-0.88

0.03

TM

240

L

S.Malt LGRMM

-0.79

0.64

TM

241

G

G

-0.77

0.02

TM

242

R

R

-0.86

0.16

IN

243

M

M

-0.93

0.50

IN

244

M

M

-0.98

0.11

IN

245

M

M

-0.59

0.77

IN

246

K

(P.Aer) KYRD+

-0.51

0.90

IN

247

Y

Y

-0.30

0.91

IN

248

R

R

-0.01

0.03

IN

249

D

(myelo) +Q A   I ERL

0.36

0.03

IN

250

Q

(P.Aer) QRAG

0.75

0.03

IN

251

R

R

0.65

0.09

IN

252

A

A

0.85

0.08

IN

253

G

(hspd1)  GK+ E  VI T +  M          IE  IQ (P.Aer) GKI ERLV

0.82

0.04

IN

254

K

K

0.63

0.28

IN

255

I

I

0.13

0.82

IN

256

S

S

-0.26

0.02

IN

257

E

(P.Aer) ERLV

-0.62

0.03

IN

258

R

R

-0.65

0.43

IN

259

L

(TNF) LV+ SE

-0.70

0.84

IN

260

V

V

-0.47

0.11

IN

261

I

I

-0.71

0.14

IN

262

T

T

-1.00

0.04

IN

263

S

S

-0.97

0.04

IN

264

E

E

-0.54

0.06

IN

265

M

M

-0.47

0.95

IN

266

I

S.Malt +IENIQ

-0.11

0.46

IN

267

E

E

-0.10

0.02

IN

268

N

N

-0.25

0.02

IN

269

I

I

-0.27

0.14

IN

270

Q

Q

-0.23

0.02

IN

271

S

S

-0.14

0.03

IN

272

V

V

-0.21

0.94

IN

273

K

K

-0.54

0.94

IN

274

A

A

-0.25

0.80

IN

275

Y

GAD65 Y WE  +  + + E L   QT LK   K    RYFN

-0.17

0.51

IN

276

C

C

-0.20

0.02

IN

277

W

W

-0.21

0.90

IN

278

E

E

-0.21

0.02

IN

279

E

E

-0.14

0.03

IN

280

A

A

-0.17

0.04

IN

281

M

M

-0.36

0.19

IN

282

E

Calgran EK IE   +

-0.09

0.02

IN

283

K

K

-0.18

0.53

IN

284

M

M

-0.56

0.21

IN

285

I

Adren ++NL Q E

-0.55

0.06

IN

286

E

E

-0.37

0.02

IN

287

N

N

-0.37

0.03

IN

288

L

(myelo) LR   LKL RK

-0.19

0.68

IN

289

R

R

-0.32

0.45

IN

290

Q

Q

-0.06

0.03

IN

291

T

(P.Aer) TELKL

-0.40

0.09

IN

292

E

E

-0.40

0.03

IN

293

L

(myelo) LKL RK

-0.13

0.97

IN

294

K

K

-0.05

0.35

IN

295

L

L

-0.13

0.83

IN

296

T

(prot) TR A YV

-0.24

0.03

IN

297

R

R

-0.49

0.35

IN

298

K

K

-0.37

0.46

IN

299

A

(P.Aer) AAYV-Y

-0.46

0.09

IN

300

A

Adren A  RYF     F

-0.29

0.06

IN

301

Y

Y

-0.60

0.57

IN

302

V

V

-0.52

0.72

IN

303

R

(P.Aer) R+FNSS (GAD65) RYFN

-0.52

0.15

IN

304

Y

Y

-0.39

0.96

TM

305

F

(mucin) FNSS

-0.42

0.23

TM

306

N

Adren N SAF

-0.65

0.02

TM

307

S

S

-0.81

0.03

TM

308

S

S

-1.05

0.17

TM

309

A

A

-0.87

0.29

TM

310

F

F

-0.60

0.96

TM

311

F

S.Malt FFSGF

-1.00

0.53

TM

312

F

F

-1.47

0.08

TM

313

S

S

-1.92

0.02

TM

314

G

(P.Aer) GFFVVFL

-2.20

0.05

TM

315

F

F

-2.24

0.45

TM

316

F

F

-2.29

0.44

TM

317

V

V

-2.06

0.29

TM

318

V

V

-2.21

0.95

TM

319

F

F

-2.43

0.92

TM

320

L

(mucin) LSV P

-2.11

0.61

TM

321

S

S

-1.85

0.04

TM

322

V

(P.Aer)  V-PYAL

-1.54

0.85

TM

323

L

L

-1.54

0.83

TM

324

P

P

-1.41

0.03

TM

325

Y

Y

-1.05

0.93

TM

326

A

(P.Aer) A-IKGI [ALIK I]

-0.99

0.11

TM

327

L

L

-1.15

0.44

EXT

328

I

I

-1.26

0.25

EXT

329

K

K

-1.66

0.46

EXT

330

G

G

-1.68

0.03

EXT

331

I

I

-1.74

0.62

EXT

332

I

I

-1.69

0.17

EXT

333

L

L

-1.72

0.32

EXT

334

R

R

-1.73

0.62

EXT

335

K

(P.Aer)  KIFTTIS

-1.68

0.92

EXT

336

I

I

-1.61

0.10

EXT

337

F

F

-1.33

0.06

EXT

338

T

T

-1.36

0.02

EXT

339

T

T

-1.45

0.06

EXT

340

I

I

-1.83

0.96

EXT

341

S

S

-1.89

0.07

EXT

342

F

F

-2.04

0.92

EXT

343

C

(S.Aur) CIVL

-2.09

0.03

EXT

344

I

I

-2.38

0.50

EXT

345

V

V

-2.22

0.13

EXT

346

L

L

-2.36

0.75

EXT

347

R

R

-1.86

0.47

EXT

348

M

M

-1.67

0.89

EXT

349

A

A

-1.05

0.08

EXT

350

V

V

-0.87

0.07

EXT

351

T

(prot) TR FP S.Malt TRQFP S.Aurand P.Aer  TR+FP

-0.34

0.03

EXT

352

R

P.Aer RQFP

-0.44

0.39

EXT

353

Q

Q

-0.09

0.03

EXT

354

F

F

-0.11

0.42

EXT

355

P

P

0.18

0.02

EXT

356

W

W

0.17

0.72

EXT

357

A

(P.Aer) AVQTW

0.00

0.05

EXT

358

V

V

-0.18

0.93

EXT

359

Q

Q

0.12

0.12

EXT

360

T

T

0.14

0.38

EXT

361

W

W

0.15

0.97

EXT

362

Y

Y

0.11

0.97

EXT

363

D

(HSPD1) DSL   N  Q         TL (TNF)  D L A IN   D+L

0.16

0.04

EXT

364

S

S

-0.11

0.02

EXT

365

L

L

-0.13

0.38

EXT

366

G

(Mucin) G IN ++D    F Q   YKT E    YNLT  +V

0.06

0.05

EXT

367

A

A

-0.09

0.07

EXT

368

I

I

-0.19

0.27

EXT

369

N

N

-0.23

0.03

EXT

370

K

K

-0.32

0.28

EXT

371

I

I

-0.65

0.88

EXT

372

Q

Calgran  Q+FL K E K    N    E +ME+

-0.57

0.10

EXT

373

D

D

-0.32

0.03

EXT

374

F

(GAD65) FLQ      L+Y

-0.22

0.77

EXT

375

L

L

-0.07

0.38

EXT

376

Q

Q

0.08

0.03

EXT

377

K

K

0.08

0.77

EXT

378

Q

Q

0.06

0.29

EXT

379

E

E

0.08

0.03

EXT

380

Y

(mucin) YKT E    YNLT  +V

0.47

0.80

EXT

381

K

K

0.28

0.42

EXT

382

T

T

0.27

0.04

EXT

383

L

L

-0.13

0.88

EXT

384

E

E

-0.21

0.03

EXT

385

Y

(mucin)YNLT  +V P.Aer  YN LTTT    +V M S.Aur Y LT T+VV S.Malt YN T  EVV

-0.10

0.97

EXT

386

N

P.Aer NL TTE  VV S.Aur NLTTTE S.Malt NLTTT and NLTT E++

-0.03

0.03

EXT

387

L

(hspd1) LTT EVV P.Aer  L TT+VV S.Aur LTTT+

0.04

0.71

EXT

388

T

P.Aer and S.Malt TTTEV S.Aur TT  VVM S.Malt +TTTEV

0.09

0.03

EXT

389

T

P.Aer and S.Aur and S.Malt TTEVV

-0.20

0.08

EXT

390

T

P.Aer TEVVM S.Aur TEV+M S.Malt TE  VV M

-0.23

0.08

EXT

391

E

P.Aer and S.Aur EVVM S.Malt EV+M

-0.27

0.04

EXT

392

V

V

0.06

0.82

EXT

393

V

V

-0.10

0.16

EXT

394

M

M

-0.16

0.70

EXT

395

E

E

-0.33

0.02

EXT

396

N

N

-0.66

0.08

EXT

397

V

V

-0.68

0.15

EXT

398

T

T

-0.33

0.06

EXT

399

A

A

0.03

0.20

EXT

400

F

F